pich47751 grutzner (Addgene inc)
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pich47751 grutzner
Pich47751 Grutzner, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pich47751/pICSL01009%3A%3AAtU6p+(Plasmid+%2346968)/pm41923635-203-175-179
Average 94 stars, based on 34 article reviews
Pich47751 Grutzner, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pich47751/pICSL01009%3A%3AAtU6p+(Plasmid+%2346968)/pm41923635-203-175-179
Average 94 stars, based on 34 article reviews
pich47751 grutzner - by Bioz Stars,
2026-09
94/100 stars
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Mutagenesis:Article Title: Species-specific PHYTOCHROME-INTERACTING FACTOR utilization in the plant morphogenetic response to environmental stimuli Article Snippet: To produce each of the gRNAs, a PCR was carried out with a primer containing the gRNA sequence and a universal primer , using the plasmid pICH86966:: AtU6p::gRNA_PDS (Addgene plasmid 46966) as a template. .. To generate the slpifq- quadruple mutant plant (carrying the mutant alleles slpif4 CR-1 , slpif7a CR-1 , slpif7b CR-1 , and slpif8a CR-1 ), each gRNA was first assembled into Level 1 vectors together with pICSL01009_U6pro (Addgene plasmid 46968) using BsaI enzyme. gRNA1 and gRNA8 were cloned into Article Title: Species-specific PHYTOCHROME-INTERACTING FACTOR utilization in the plant morphogenetic response to environmental stimuli. Article Snippet: PHYTOCHROME-INTERACTING FACTORs (PIFs) regulate growth-related gene expression in response to environmental conditions.. Among their diverse functions in regulating signal responses, PIFs play an important role in thermomorphogenesis (the response to increased ambient temperature) and in the shade avoidance response.. While numerous studies have examined the varied roles of PIFs in Arabidopsis (Arabidopsis thaliana), their roles in crop plants remain poorly investigated. Plasmid Preparation:Article Title: Species-specific PHYTOCHROME-INTERACTING FACTOR utilization in the plant morphogenetic response to environmental stimuli Article Snippet: To produce each of the gRNAs, a PCR was carried out with a primer containing the gRNA sequence and a universal primer , using the plasmid pICH86966:: AtU6p::gRNA_PDS (Addgene plasmid 46966) as a template. .. To generate the slpifq- quadruple mutant plant (carrying the mutant alleles slpif4 CR-1 , slpif7a CR-1 , slpif7b CR-1 , and slpif8a CR-1 ), each gRNA was first assembled into Level 1 vectors together with pICSL01009_U6pro (Addgene plasmid 46968) using BsaI enzyme. gRNA1 and gRNA8 were cloned into Article Title: Fine tuning wheat heading time through genome editing of transcription factor binding sites in Ppd-1 gene promoter. Article Snippet: .. Construction of plasmid for gene editing To create plasmid for the biobalistic transformation of wheat we used plasmids from MoClo Kit: pFH85 (Addgene plasmid #128198; https://www.addgene.org/128198/), pFH33 (Addgene plasmid #128198), Article Title: Supporting Information for Null mutants of a tomato Rho of Plants (ROP) exhibit enhanced water use efficiency without a penalty to yield Article Snippet: .. Plasmids used in this study Name Description E. coli A. tumefacien s Plant Source Gateway vectors 1 pENTR221 Kan Lab stocks 2 pDEST22 Amp 3 pDEST32 Gent 4 pENTRp4-p1R-35S Kan 5 pENTR221-GFP Kan 6 pSYSl02 pENTRp2rp3-SlROP9 Kan 7 pk7m34GW Kan 8 pSYSl10 pk7-35S -GFP-SlROP9 Spec Spec + Gent Kan This work 9 pENTR221-YN Kan Lab stocks 10 pENTRp2rp3-YC Kan Lab stocks 11 pSYSl07 pk7-35S-YN-SlROP9 Spec Spec + Gent Kan This work 12 pSYSl28 pENTR221-SlICR1 without stop codon Kan 13 pSYSl29 pENTR221-SlICR2 without stop codon Kan 11 pSYSl30 pk7-35S-SlICR1-YC Spec Spec + Gent Kan 14 pSYSl31 pk7-35S-SlICR2-YC Spec Spec + Gent Kan 15 pk7-35S-AtICR1-YC Spec Spec + Gent Kan Lab stocks Plasmids for CRISPR-cas9 16 pICH86966 (SgRNA backbone) Addgene 17 pICSL01009 (AtU6 promoter) 18 Article Title: PLETHORA transcription factors promote early embryo development through induction of meristematic potential. Article Snippet: .. The in vitro translation vector pSPUTK-GG was designed by making the Gateway vector pSPUTK (Invitrogen) Golden Gate-compatible. pSPUTK amplicons were amplified with pSPUTK F1/R1 and pSPUTK F2/R2, and combined with a lacZ amplicon originating from Article Title: Species-specific PHYTOCHROME-INTERACTING FACTOR utilization in the plant morphogenetic response to environmental stimuli. Article Snippet: PHYTOCHROME-INTERACTING FACTORs (PIFs) regulate growth-related gene expression in response to environmental conditions.. Among their diverse functions in regulating signal responses, PIFs play an important role in thermomorphogenesis (the response to increased ambient temperature) and in the shade avoidance response.. While numerous studies have examined the varied roles of PIFs in Arabidopsis (Arabidopsis thaliana), their roles in crop plants remain poorly investigated. Article Title: Fine tuning wheat heading time through genome editing of transcription factor binding sites in Ppd-1 gene promoter Article Snippet: .. To create plasmid for the biobalistic transformation of wheat we used plasmids from MoClo Kit: pFH85 (Addgene plasmid #128198; https://www.addgene.org/128198/ ), pFH33 (Addgene plasmid #128198), Article Title: PLETHORA transcription factors promote early embryo development through induction of meristematic potential Article Snippet: .. The in vitro translation vector pSPUTK-GG was designed by making the Gateway vector pSPUTK (Invitrogen) Golden Gate-compatible. pSPUTK amplicons were amplified with pSPUTK F1/R1 and pSPUTK F2/R2, and combined with a lacZ amplicon originating from Clone Assay:Article Title: Species-specific PHYTOCHROME-INTERACTING FACTOR utilization in the plant morphogenetic response to environmental stimuli Article Snippet: To produce each of the gRNAs, a PCR was carried out with a primer containing the gRNA sequence and a universal primer , using the plasmid pICH86966:: AtU6p::gRNA_PDS (Addgene plasmid 46966) as a template. .. To generate the slpifq- quadruple mutant plant (carrying the mutant alleles slpif4 CR-1 , slpif7a CR-1 , slpif7b CR-1 , and slpif8a CR-1 ), each gRNA was first assembled into Level 1 vectors together with pICSL01009_U6pro (Addgene plasmid 46968) using BsaI enzyme. gRNA1 and gRNA8 were cloned into Article Title: Species-specific PHYTOCHROME-INTERACTING FACTOR utilization in the plant morphogenetic response to environmental stimuli. Article Snippet: PHYTOCHROME-INTERACTING FACTORs (PIFs) regulate growth-related gene expression in response to environmental conditions.. Among their diverse functions in regulating signal responses, PIFs play an important role in thermomorphogenesis (the response to increased ambient temperature) and in the shade avoidance response.. While numerous studies have examined the varied roles of PIFs in Arabidopsis (Arabidopsis thaliana), their roles in crop plants remain poorly investigated. Transformation Assay:Article Title: Fine tuning wheat heading time through genome editing of transcription factor binding sites in Ppd-1 gene promoter. Article Snippet: .. Construction of plasmid for gene editing To create plasmid for the biobalistic transformation of wheat we used plasmids from MoClo Kit: pFH85 (Addgene plasmid #128198; https://www.addgene.org/128198/), pFH33 (Addgene plasmid #128198), Article Title: Fine tuning wheat heading time through genome editing of transcription factor binding sites in Ppd-1 gene promoter Article Snippet: .. To create plasmid for the biobalistic transformation of wheat we used plasmids from MoClo Kit: pFH85 (Addgene plasmid #128198; https://www.addgene.org/128198/ ), pFH33 (Addgene plasmid #128198), CRISPR:Article Title: Supporting Information for Null mutants of a tomato Rho of Plants (ROP) exhibit enhanced water use efficiency without a penalty to yield Article Snippet: .. Plasmids used in this study Name Description E. coli A. tumefacien s Plant Source Gateway vectors 1 pENTR221 Kan Lab stocks 2 pDEST22 Amp 3 pDEST32 Gent 4 pENTRp4-p1R-35S Kan 5 pENTR221-GFP Kan 6 pSYSl02 pENTRp2rp3-SlROP9 Kan 7 pk7m34GW Kan 8 pSYSl10 pk7-35S -GFP-SlROP9 Spec Spec + Gent Kan This work 9 pENTR221-YN Kan Lab stocks 10 pENTRp2rp3-YC Kan Lab stocks 11 pSYSl07 pk7-35S-YN-SlROP9 Spec Spec + Gent Kan This work 12 pSYSl28 pENTR221-SlICR1 without stop codon Kan 13 pSYSl29 pENTR221-SlICR2 without stop codon Kan 11 pSYSl30 pk7-35S-SlICR1-YC Spec Spec + Gent Kan 14 pSYSl31 pk7-35S-SlICR2-YC Spec Spec + Gent Kan 15 pk7-35S-AtICR1-YC Spec Spec + Gent Kan Lab stocks Plasmids for CRISPR-cas9 16 pICH86966 (SgRNA backbone) Addgene 17 pICSL01009 (AtU6 promoter) 18 In Vitro:Article Title: PLETHORA transcription factors promote early embryo development through induction of meristematic potential. Article Snippet: .. The in vitro translation vector pSPUTK-GG was designed by making the Gateway vector pSPUTK (Invitrogen) Golden Gate-compatible. pSPUTK amplicons were amplified with pSPUTK F1/R1 and pSPUTK F2/R2, and combined with a lacZ amplicon originating from Article Title: PLETHORA transcription factors promote early embryo development through induction of meristematic potential Article Snippet: .. The in vitro translation vector pSPUTK-GG was designed by making the Gateway vector pSPUTK (Invitrogen) Golden Gate-compatible. pSPUTK amplicons were amplified with pSPUTK F1/R1 and pSPUTK F2/R2, and combined with a lacZ amplicon originating from Amplification:Article Title: PLETHORA transcription factors promote early embryo development through induction of meristematic potential. Article Snippet: .. The in vitro translation vector pSPUTK-GG was designed by making the Gateway vector pSPUTK (Invitrogen) Golden Gate-compatible. pSPUTK amplicons were amplified with pSPUTK F1/R1 and pSPUTK F2/R2, and combined with a lacZ amplicon originating from Article Title: PLETHORA transcription factors promote early embryo development through induction of meristematic potential Article Snippet: .. The in vitro translation vector pSPUTK-GG was designed by making the Gateway vector pSPUTK (Invitrogen) Golden Gate-compatible. pSPUTK amplicons were amplified with pSPUTK F1/R1 and pSPUTK F2/R2, and combined with a lacZ amplicon originating from Cloning:Article Title: PLETHORA transcription factors promote early embryo development through induction of meristematic potential. Article Snippet: .. The in vitro translation vector pSPUTK-GG was designed by making the Gateway vector pSPUTK (Invitrogen) Golden Gate-compatible. pSPUTK amplicons were amplified with pSPUTK F1/R1 and pSPUTK F2/R2, and combined with a lacZ amplicon originating from Article Title: PLETHORA transcription factors promote early embryo development through induction of meristematic potential Article Snippet: .. The in vitro translation vector pSPUTK-GG was designed by making the Gateway vector pSPUTK (Invitrogen) Golden Gate-compatible. pSPUTK amplicons were amplified with pSPUTK F1/R1 and pSPUTK F2/R2, and combined with a lacZ amplicon originating from |